Composite
Part:BBa_K5097008:Design
Designed by: Kelly Acen, Marie Box, Alexia Darby, Jena Dookie, Blake Dieckman, Runya Chaora, Chris Dorce, Adrianna Dugan, Elber Lopez-Hernandez, kayla VanPelt, Makenna Ventuleth Group: iGEM24_Oneonta (2024-09-28)
pASR-RFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 707
Illegal AgeI site found at 819 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Along with pARS and RFP, a strong ribosome binding site (BBa_B0034) was added.
Source
This promoter is a pH-responsive promoter native to E.coli. It induces transcription in acidic conditions.