Composite

Part:BBa_K5097008:Design

Designed by: Kelly Acen, Marie Box, Alexia Darby, Jena Dookie, Blake Dieckman, Runya Chaora, Chris Dorce, Adrianna Dugan, Elber Lopez-Hernandez, kayla VanPelt, Makenna Ventuleth   Group: iGEM24_Oneonta   (2024-09-28)


pASR-RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
    Illegal AgeI site found at 819
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Along with pARS and RFP, a strong ribosome binding site (BBa_B0034) was added.


Source

This promoter is a pH-responsive promoter native to E.coli. It induces transcription in acidic conditions.

References